Invitrogen™ Human MMP-12 ELISA Kit

Catalog No.	Quantity
EH327RB	96 Tests
EH327RBX5	5 x 96 Tests
EH327RBX10	10 x 96 Tests

Catalog No. EH327RB Supplier Invitrogen™ Supplier No. EH327RB

Description

ELISA

Human MMP-12 quantitates human MMP-12 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human MMP-12.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.

Specifications

• Accession Number: P39900
• Assay Range: 0.04-10 ng/mL
• Assay Sensitivity: 0.04 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 3.4.24.65; AV378681; HME; Macrophage elastase; macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; ME; MGC138506; MME; Mmel; MMP; Mmp12; MMP-12; MMPs3.4.24.65; AV378681; HME; Macrophage elastase; macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; ME; MGC138506; MME; Mmel; MMP; Mmp12; MMP-12; MMPs;
• Gene ID (Entrez): 4321
• Gene Symbol: Mmp12
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: MMP12
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov