Gibco™ Human/Mouse/Rat BMP-2 Recombinant Protein, PeproTech®

Catalog No.	Quantity
12002C10UG	10 μg
120-02C-1MG	1 mg
12002C250UG	250 μg
120-02C-2UG	2 μg
12002C500UG	500 μg
12002C50UG	50 μg

Catalog No. 12002C10UG Supplier Gibco™ Supplier No. 12002C10UG

Description

Recombinant Protein

Recombinant Human/Murine/Rat BMP-2 derived from CHO cells is a homodimeric glycoprotein that consists of two 114 amino acid polypeptide chains linked by a single disulfide bond. Due to glycosylation, CHO cell-derived Human/Murine/Rat BMP-2 migrates at an apparent molecular weight of approximately 28-29 kDa by SDS-PAGE analysis under non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (Hogan 1996, Reddi 1998 and Francis-West et al. 1999). Mature BMPs are 30-38 kDa proteins that assume a TGF-beta -like cysteine knot configuration. Lovostatin increases bone formation by turning on the bmp-2 gene (Mundy et al. 1999). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (Macias et al. 1999, Lecanda et al. 1997). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (Hogan 1996, Dale and Wardle 1999). BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (Massague 1994, Derynck 1997, Attisano 1993).

Specifications

• Accession Number: P12643, P21274, P49001
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 12156, 29373, 650
• Molecular Weight (g/mol): 25.8 kDa
• Name: Human/Mouse/Rat BMP-2
• Quantity: 10 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: 2610024H22Rik; AI467020; AL117858; AW546137; BB189135; BDA2; BMP; BMP2; BMP-2; BMP2A; BMP-2 A; BMPRII; BMPR-II; Bone morphogenetic protein; Bone morphogenetic protein 2; bone morphogenetic protein 2 precursor (BMP-2) (BMP-2 A); bone morphogenetic protein 2 A; H-BMP-2; morphogen
• Common Name: BMP-2
• Gene Symbol: BMP2
• Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 40-100 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.