Gibco™ Human/Mouse/Rat Myostatin Recombinant Protein, PeproTech®

Description

Recombinant Protein

The amino acid sequence of mature myostatin is extremely conserved across species, and is the same in murine, rat, chicken, turkey, porcine, and human. Myostatin is expressed as the C-terminal part of a precursor polypeptide, which also contains a short N-terminal signal sequence for secretion, and a propeptide of 243 amino acids. After dimerization of this precursor, the covalent bonds between the propeptide and the mature ligand are cleaved by furin-type proteases. However, the resulting two proteins remain associated through non-covalent interactions, and are secreted as a latent complex. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The protein encoded by this gene is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.

Specifications

• Accession Number: O08689, O14793, O35312
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 17700, 2660, 29152
• Molecular Weight (g/mol): 25 kDa
• Name: Human/Mouse/Rat Myostatin
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: Cmpt; compact; Gdf8; GDF-8; growth differentiation factor 8; growth/differentiation factor 8; H-Myo-Propeptide; H-Myostatin; MSLHP; MSTN; Myostatin
• Common Name: Myostatin
• Gene Symbol: Mstn
• Biological Activity: Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.