Invitrogen™ Human MR-ProADM ELISA Kit

Catalog No.	Quantity
EEL189	96 Tests

Catalog No. EEL189 Supplier Invitrogen™ Supplier No. EEL189

Description

ELISA

The Human Mid regional pro adrenomedullin ELISA Kit (MR ProADM) ELISA quantitates MR ProADM in serum, plasma and other biological fluids. Principle of the method The Human Mid regional pro adrenomedullin (MR ProADM) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

MR-proADM, short for mid-regional proadrenomedullin, is a precursor protein that gives rise to the adrenomedullin (ADM) hormone. It is involved in various physiological processes, particularly in the regulation of cardiovascular function and inflammation. Functionally, MR-proADM is associated with vasodilation, blood pressure regulation, and immune response modulation. It acts as a biomarker for endothelial dysfunction and can be used to assess the severity of certain cardiovascular diseases. Elevated levels of MR-proADM have also been linked to systemic inflammation and sepsis. Structurally, MR-proADM is a protein that consists of a chain of amino acids. It undergoes post-translational processing to generate the active form of ADM, which is a shorter peptide consisting of 52 amino acids. Localization-wise, MR-proADM is primarily produced in various tissues, including the adrenal glands, lungs, and endothelial cells lining blood vessels. It is released into the bloodstream and can be measured as a biomarker to provide insight into cardiovascular health and inflammatory conditions.

Specifications

• Accession Number: P35318
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Mid-regional proadrenomedullinMid-regional proadrenomedullin;
• Gene ID (Entrez): 133
• Gene Symbol: ADM
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: MR-proADM
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min