Invitrogen™ Human MUC5AC ELISA Kit

Catalog No.	Quantity
EEL062	96 Tests

Catalog No. EEL062 Supplier Invitrogen™ Supplier No. EEL062

Description

ELISA

The Human Mucin 5 Subtype AC (MUC5AC) ELISA quantitates MUC5AC in serum, plasma, and other biological fluids The Human Mucin 5 Subtype AC (MUC5AC) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

Specifications

• Accession Number: P98088
• Assay Range: 0.31-20 ng/mL
• Assay Sensitivity: 0.19 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Mucin
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 2210005L13Rik; gastric mucin; LeB; lewis B blood group antigen; LOW QUALITY PROTEIN: mucin-5AC; major airway glycoprotein; MGM; MUC5; MUC5AC; MUC-5AC; mucin; mucin 5, subtypes A and C; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5ac; mucin 5AC, oligomeric mucus/gel-forming; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin-5 subtype AC, tracheobronchial; Mucin-5AC; TBM; Tracheobronchial mucin2210005L13Rik; gastric mucin; LeB; lewis B blood group antigen; LOW QUALITY PROTEIN: mucin-5AC; major airway glycoprotein; MGM; MUC5; MUC5AC; MUC-5AC; mucin; mucin 5, subtypes A and C; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5ac; mucin 5AC, oligomeric mucus/gel-forming; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin-5 subtype AC, tracheobronchial; Mucin-5AC; TBM; Tracheobronchial mucin;
• Gene ID (Entrez): 4586
• Gene Symbol: MUC5AC
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: MUC5AC
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min