Molecular Probes™ Human Neural Stem Cell Immunocytochemistry Kit

Catalog No.	Quantity
A24354	20 Tests

Catalog No. A24354 Supplier Molecular Probes™ Supplier No. A24354

Description

Human Neural Stem Cell Immunocytochemistry Kit

The Molecular Probes™ Human Neural Stem Cell Immunocytochemistry Kit enables convenient image-based analysis of four common markers of human neural stem cells (NSCs): nestin, PAX6, SOX1, and SOX2. This complete immunocytochemistry (ICC) kit includes an optimized set of primary and secondary antibodies, a nuclear DNA stain, and all of the buffers necessary to complete the staining protocol.

Easily scalable to any culture plate, dish, or slide format, the Human Neural Stem Cell Immunocytochemistry Kit comes with reagents sufficient to run 20 samples using a 50μL staining volume.

Confirm expression of key neural stem cell markers with highly specific antibodies

Get more information per sample by measuring more than one marker at a time

Generate beautiful multiplexed images with minimal effort

Process samples with confidence using a complete & optimized set of ICC reagents

Confirm Expression of NSC Markers
The antibodies included in the kit have been carefully selected for high performance in ICC analysis of NSCs. These antibodies are well suited for the characterization of NSCs generated using Gibco™ PSC Neural Induction Medium.

Get More Information Per Sample
Save time and precious sample. The antibody combinations in this kit have been designed to allow for simultaneous assessment of two markers at a time (e.g., nestin & SOX2 or SOX1 & PAX6) along with nuclear DNA staining.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of NSC marker expression using commonly available blue, green, and orange/red filter sets.

Process Samples with Confidence
Pre-tested fixation, permeabilization, blocking, and wash buffers allow you to focus on answering scientific questions rather than spending time determining reagent compatibility.

Specifications

• Conjugate: Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594
• Quantity: 20 Tests
• Content And Storage: Contains:
  20 μL of Goat Anti-Human SOX1 (50X)
  20 μL of Rabbit Anti-Human SOX2 (50X)
  20 μL of Mouse Anti-Human Nestin (50X)
  20 μL of Rabbit Anti-Human PAX6 (50X)
  20 μL of Alexa Fluor™ 488 Anti-Mouse (250X)
  20 μL of Alexa Fluor™ 488 Anti-Goat (250X)
  40 μL of Alexa Fluor™ 555 Anti-Rabbit (250X)
  40 μL of Alexa Fluor™ 594 Anti-Rabbit (250X)
  1 vial of NucBlue™ Fixed Cell Stain ReadyProbes™ Reagent (1X)
  10 mL of Fixative Solution (1X)
  10 mL of Permeabilization Solution (1X)
  20 mL of Blocking Solution (1X)
  20 mL of Wash Buffer (10X)
• Product Type: Immunocytochemistry Kit

Frequently Asked Questions (FAQs)

Can I purchase the primary antibodies from the Human Neural Stem Cell Immunocytochemistry Kit (Cat. No. A24354) separately?

Yes, the primary antibodies from the Human Neural Stem Cell Immunocytochemistry Kit (Cat. No. A24354) can be purchased separately. Here are the catalog numbers:

• Nestin Monoclonal Antibody (10C2), eBioscience (Cat. No. 14984382)
• SOX2 Polyclonal Antibody (Cat. No. 481400)
• PAX6 Polyclonal Antibody (Cat. No. 426600)
• SOX1 Goat anti-Human, Mouse, Rat, Polyclonal, R&D Systems; available through Fisher Scientific (Cat. No. AF3369)

The concentrations of the antibodies in the Human Neural Stem Cell Immunocytochemistry Kit are proprietary. If you purchase the above antibodies separately, you will need to determine the best concentrations to use in your assay.

The Human Neural Stem Cell Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain?

If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

Can I use the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis?

We do not recommend using the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Do you offer the individual components of the Human Neural Stem Cell Immunocytochemistry Kit as standalone products?

Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.