Invitrogen™ Human NF-L ELISA Kit

Catalog No.	Quantity
BMS2362	96 Tests
BMS2362TEN	10 x 96 Tests

Catalog No. BMS2362 Supplier Invitrogen™ Supplier No. BMS2362

Description

ELISA

The Human NF-L ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human NF-L in CSF, serum, and plasma (citrate and heparin) and cell culture supernatants (CCS).

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

Specifications

• Accession Number: P07196, P08551, P19527
• Assay Range: 1.6-1200
• Assay Sensitivity: 0.8 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cerebrospinal Fluid, Plasma, Serum, Supernatant
• Target Species: Human, Mouse, Rat
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L;
• Gene ID (Entrez): 18039, 4747, 83613
• Gene Symbol: NEFL
• Interassay CV: 4%
• Intraassay CV: 3.7%
• Kit Contents: Pre-coated microwell plate, Standard, Biotinylated Detection Antibody, Streptavidin-HRP, Sample Diluent, Wash Buffer Concentrate, Assay Buffer concentrate, Stop Solution, Substrate Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: NF-L
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 45 min