Invitrogen™ Human NGF Recombinant Protein

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

Specifications

• Accession Number: P01138
• For Use With (Application): ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 40 mM arginine, 40 mM histidine with 0.01% Tween 80, 150 mM NaCl, 5% mannitol, 5% trehalose and no preservative; pH 5.5
• Gene ID (Entrez): 4803
• Molecular Weight (g/mol): 13.2 kDa
• Name: Human NGF
• Quantity: 5 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/ μg
• Gene Alias: B nerve growth factor; beta ngf; beta-nerve growth factor; BetaNGF; beta-NGF; bNGF; b-NGF; HSAN5; MGC161426; MGC161428; nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta; nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; NGF; NGF b; NGF β; Ngfb; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
• Common Name: NGF
• Gene Symbol: NGF
• Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/mL
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Human β-NGF, amino acids Ser122-Arg239 (Accession # NP_002497.2)
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: >95% by SDS-PAGE