Invitrogen™ Human Nogo-A/Reticulon 4A ELISA Kit

Catalog No.
EH343RBX10	10 x 96 Tests; HRP

Catalog No. EH343RBX10 Supplier Invitrogen™ Supplier No. EH343RBX10

Description

ELISA

Human Nogo-A/Reticulon 4A quantitates human Nogo-A/Reticulon 4A in supernatant. The assay will exclusively recognize both natural and recombinant human Nogo-A/Reticulon 4A.

NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS, blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.

Specifications

• Accession Number: Q9NQC3
• Assay Range: 0.42-100 ng/mL
• Assay Sensitivity: 0.42 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; Foocen; glut4 vesicle 20 kDa protein; GLUT4 vesicle 20kDa protein; Human NogoA; KIAA0886; mKIAA0886; mKIAA4153; My043; My043 protein; Nbla00271; Nbla10545; neurite growth inhibitor 220; neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; NgA; NI220/250; NI-220/250; NI-250; Nogo; Nogo protein; Nogo-A; Nogo-B; NOGOC; Nogo-C; NSP; NSP-CL; r; rat N; rat NogoA; reticulon 4; reticulon 5; reticulon-4; Reticulon-5; RTN4; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; RTN-x; SP1507; Vp201110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; Foocen; glut4 vesicle 20 kDa protein; GLUT4 vesicle 20kDa protein; Human NogoA; KIAA0886; mKIAA0886; mKIAA4153; My043; My043 protein; Nbla00271; Nbla10545; neurite growth inhibitor 220; neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; NgA; NI220/250; NI-220/250; NI-250; Nogo; Nogo protein; Nogo-A; Nogo-B; NOGOC; Nogo-C; NSP; NSP-CL; r; rat N; rat NogoA; reticulon 4; reticulon 5; reticulon-4; Reticulon-5; RTN4; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; RTN-x; SP1507; Vp20;
• Gene ID (Entrez): 57142
• Gene Symbol: Rtn4
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Nogo-A
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min