Invitrogen™ Human Osteocalcin Instant ELISA™ Kit

Catalog No. 50-178-97

Supplier: Invitrogen™ BMS2020INST

Description

ELISA

The Human Osteocalcin ELISA quantitates Hu Osteocalcin in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu Osteocalcin. Principle of the method The Human Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Specifications

• Accession Number: P02818
• Assay Range: 1.2 to 75 ng/mL
• Assay Sensitivity: 0.2 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 632
• Gene Symbol: BGP,OC,OCN
• Interassay CV: 8.1%
• Intraassay CV: 8.3
• Kit Contents: Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 25 μL, Serum 25 μL, Supernatant 25 μL
• Storage Requirements: -20°C
• Synonym: Gamma-carboxyglutamic acid-containing protein
• Target: Osteocalcin
• Test Time: 15 mins
• Total Assay Time: 2 hrs 10 mins