Invitrogen™ Human OX40L ELISA Kit

Description

ELISA

Human OX40L quantitates human OX40L in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human OX40L.

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

Specifications

• Accession Number: Ath1; Ath-1; atherosclerosis 1; CD134 ligand; CD134L; CD252; Glycoprotein Gp34; GP34; O x 40 antigen ligand; O x 40 ligand; O x 40 L; OX-40 L; O x 4 OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34 kD); tax-transcriptionally activated glycoprotein 1 ligand; Tnfsf4; TNLG2B; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand 2 B; tumor necrosis factor ligand superfamily member 4; tumor necrosis factor superfamily member 4; Txgp1; Txgp1l
• Assay Range: 61 to 15,000 pg/mL
• Assay Sensitivity: 61 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 7292
• Gene Symbol: Tnfsf4
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: OX40L (CD252)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins