Invitrogen™ Human Oxidized LDL ELISA Kit

Catalog No.	Quantity
EEL074	96 Tests

Catalog No. EEL074 Supplier Invitrogen™ Supplier No. EEL074

Description

ELISA

The Human Oxidized Low Density Lipoprotein (OxLDL) ELISA quantitates OxLDL in serum, plasma, and other biological fluids The Human Oxidized Low Density Lipoprotein (OxLDL) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Low density lipoprotein (LDL) oxidative modification is one of the major processes involved in atherosclerosis along with other degenerative disorders. Oxidized LDL is preferentially accumulated by macrophages which become so called 'foam cells' within the atheromatous plaque and contributes to the accumulation of cholesterol, typical of atherosclerosis.

Specifications

• Assay Range: 62.5-4000 pg/mL
• Assay Sensitivity: 37.5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Apolipoproteins
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Oxidized low-density lipoprotein; Ox-LDLOxidized low-density lipoprotein; Ox-LDL;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Oxidized LDL
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min