Invitrogen™ Human P3NP ELISA Kit

Catalog No.	Quantity
EEL204	96 Tests

Catalog No. EEL204 Supplier Invitrogen™ Supplier No. EEL204

Description

ELISA

Human P3NP ELISA Kit quantifies P3NP in serum and plasma. The Human P3NP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Procollagen type III N-terminal peptide (P3NP) is released as part of collagen synthesis process in muscle. The increase of circulating P3NP is a marker not only of muscle growth, but also of muscle repair and fibrosis. P3NP may be a potential biomarker for sarcopenia.

Specifications

• Accession Number: P02461
• Assay Range: 23.44-1500 pg/mL
• Assay Sensitivity: 14.06 pg/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: PIIINP gene; Procollagen 3 N-Terminal Propeptide; Procollagen III N-Terminal PropeptidePIIINP gene; Procollagen 3 N-Terminal Propeptide; Procollagen III N-Terminal Propeptide;
• Gene ID (Entrez): 1281
• Gene Symbol: COL3A1
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis, Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Shipping Condition: Wet Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: P3NP
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min