Invitrogen™ eBioscience™ Human PAI-1 Platinum ELISA Kit 

For the quantitative detection of human PAI-1.

Manufacturer:  Invitrogen™ BMS2033

Catalog No. 50-112-5262

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human PAI-1
Biotin-Conjugate anti-human PAI-1 polyclonal antibody
Streptavidin-HRP
Human PAI-1 Standard lyophilized, 10000pg/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

Specifications

• Accession Number: P05121
• Conjugate: Biotin
• Gene ID (Entrez): 5054
• Kit Contents: Adhesive Plate Covers
• Quantity: 96 tests
• Sensitivity: 29.0 pg/mL
• Sample Size: 50μL (1:50 prediluted)
• Species: Human
• Test Time: 1 hr 20 min
• Target: Human
• Synonym: Serpin peptidase inhibitor, PAI-E, PLANH1
• Intraassay CV: 4.7%

• Assay Range: 78-5,000 pg/mL
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA Kit
• Regulatory Status: RUO
• Sample Type: Plasma,Serum,Supernatant
• Sample Volume: Plasma,10 μL|Serum,10 μL|Supernatant,10 μL
• Storage Requirements: 2-8°C
• Total Assay Time: 3 hr 10 min
• Gene Symbols: SERPINE1
• Interassay CV: 5%
• Label or Dye: HRP

For Research Use Only