Gibco™ Human PAI-1 (Serpin E1) Recombinant Protein, PeproTech®

Catalog No.	Quantity
140-04-50UG	50 μg
14004100UG	100 μg
140-04-10UG	10 μg
140-04-1MG	1 mg
14004250UG	250 μg
140-04-2UG	2 μg
14004500UG	500 μg

Catalog No. 140-04-50UG Supplier Gibco™ Supplier No. 1400450UG

Description

Recombinant Protein

Recombinant Human PAI-1 is a 42.7 kDa protein containing 379 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

Specifications

• Accession Number: P05121
• For Use With (Application): Control, Western Blot Control, ELISA Standard, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 5054
• Molecular Weight (g/mol): 42.7 kDa
• Name: Human PAI-1 (Serpin E1)
• Purification Method: Purified
• Quantity: 50 μg
• Source: E. coli
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; PAI1; PAI-1; PAI1 protein; PAI1A; Pai1aa; Planh; Planh1; plas; plasminogen activator inhibitor; plasminogen activator inhibitor 1; plasminogen activator inhibitor I; plasminogen activator inhibitor, type I; plasminogen activator inhibitor-1; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); putative plasminogen activator inhibitor-1; RATPAI1A; serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, member 1; serpin E1; serpin family E member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE1; SERPINE-1
• Common Name: PAI1
• Gene Symbol: Serpine1
• Biological Activity: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37°C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was obtained (at a concentration of 2.0 ug/ml)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAMLQLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE ISTTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIINDWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DSSTHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPYHG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLLVLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQA LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRHNPTGTV LFMGQVMEP
• Content And Storage: -20°C
• Activity: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 °C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was obtained at a concentration of 2.0 ug/ml.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.