Recombinant Protein;500UG;Human PD-1 Full Length, His Tag (PD1-H52H6) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Leu 288 (Accession # Q15116-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human PD-1 Full Length, His Tag (Cat. No. PD1-H52H6) at 0.2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.4-3 ng/mL (QC tested).;Serial dilutions of nivolumab were added into Human PD-1 Full Length, His Tag (Cat. No. PD1-H52H6) : Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.14891 (QC tested).