Invitrogen™ Human PD-L1 (B7-H1) Fc Chimera Recombinant Protein

Catalog No.	Quantity
A42498	50 μg

Catalog No. A42498 Supplier Invitrogen™ Supplier No. A42498

Description

Recombinant Protein

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

Specifications

• Accession Number: Q9NZQ7
• For Use With (Application): Control, Binding Assay, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 29126
• Molecular Weight (g/mol): 52 kDa
• Name: Human PD-L1 (B7-H1) Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Source: HEK293 cells
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
• Common Name: PD-L1 (CD274)
• Gene Symbol: Cd274
• Biological Activity: 1.Immobilized Recombinant Human PD1/PDCD1 Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Human PD-L1 (B7-H1) Fc Chimera Recombinant Protein, the EC50 is 220-660 ng/mL 2.Immobilized Human PD-L1 (B7-H1) Fc Chimera Recombinant Protein (at μg/mL (100 μL/well) can bind Recombinant Human PD1/PDCD1 Protein (Fc & AVI Tag), Biotinylated, the EC50 is 5-15 ng/m(Routinely tested).)
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human B7-H1, amino acids Met1-Thr239 (Accession # NP_054862.1) with a C-terminal human IgG1 Fc-tag
• Content And Storage: Includes 1 vial of lyophilized protein. Store at -20 °C.
• Activity: 1.Immobilized Recombinant Human PD1/PDCD1 Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Human PD-L1 (B7-H1) Fc Chimera Recombinant Protein, the EC50 is 220-660 ng/mL.<br><br>2.Immobilized Human PD-L1 (B7-H1) Fc Chimera Recombinant Protein at μg/mL (100 μL/well) can bind Recombinant Human PD1/PDCD1 Protein (Fc & AVI Tag), Biotinylated, the EC50 is 5-15 ng/m(Routinely tested).
• Endotoxin Level: < 1 EU/μg
• Expression System: HEK293 cells
• Protein Family: CD Proteins
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Apoptosis, Immunology, Inflammation, Oncology
• Purity or Quality Grade: ≥95 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.
• Protein: CD274 molecule