Invitrogen™ Human PD-L1 ELISA Kit

Description

ELISA

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

Specifications

• Accession Number: Q9NZQ7
• Assay Range: 4.69 to 300 pg/mL
• Assay Sensitivity: <0.6 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 29126
• Gene Symbol: Cd274
• Interassay CV: 3.4%
• Intraassay CV: 2.1
• Kit Contents: Pre-coated 96 well plate Standard, Biotinylated Detection antibody Sample Diluent Assay Buffer concentrate Wash Buffer concentrate, SAV-HRP Chromogen Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: PD-L1 (CD274)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs