Invitrogen™ Human PDGF-AA Recombinant Protein

Catalog No.	Quantity
PIRP8655	10 μg

Catalog No. PIRP8655 Supplier Invitrogen™ Supplier No. RP8655

Description

Recombinant Protein

Recombinant human PDGF-AA is a non-glycosylated disulfide-linked homodimer, containing two 125 amino acid chains. Reconstitute using sterile water at 0.1 mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability.

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Specifications

• Accession Number: P04085
• Concentration: 0.1 mg/mL
• For Use With (Application): Control, Bioactivity
• Formulation: 0.1% TFA with no preservative
• Gene ID (Entrez): 5154
• Molecular Weight (g/mol): 14.4 kDa
• Name: Human PDGF-AA
• Purification Method: Purified
• Quantity: 10 μg
• Source: E. coli
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: ≤0.1 EU/μg
• Gene Alias: H-PDGF-AA; PDGF A-chain; PDGF subunit A; PDGF1; PDGF-1; Pdgfa; PDGF-A; PDGFACP; platelet derived growth factor subunit A; platelet derived growth factor, alpha; Platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A; Rpa1
• Common Name: PDGF-AA
• Gene Symbol: Pdgfa
• Biological Activity: ED50 =50 ng/mL; determined by the dose-dependent proliferation of mouse 3T3 indicator cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: ED50 ≤50 ng/mL; determined by the dose-dependent proliferation of mouse 3T3 indicator cells.
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: ≥95%

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov