Gibco™ Human PDGF-AB Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in 10 mM acetic acid to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF.

Specifications

• Accession Number: P01127, P04085
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 5154, 5155
• Molecular Weight (g/mol): 25.5 kDa
• Name: Human PDGF-AB
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: Becaplermin; c-sis; H-PDGF-AB; IBGC5; PDGF A-chain; PDGF subunit A; PDGF subunit B; PDGF, B chain; PDGF1; PDGF-1; PDGF2; PDGF-2; Pdgfa; PDGF-A; PDGFAB; PDGFB; PDGF-B; platelet derived growth factor subunit A; platelet derived growth factor subunit B; platelet derived growth factor, alpha; platelet derived growth factor, B polypeptide; platelet-derived growth factor 2; platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor subunit A; Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); proto-oncogene c-Sis; SIS; SSV
• Common Name: PDGF-AB
• Biological Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human PDGF-AB recombinant protein is a disulfide-linked heterodimer containing one 125 amino acid alpha chain and one 109 amino acid beta chain
• Content And Storage: -20°C
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: PDGFs (Platelet-Derived Growth Factors)
• Research Category: Immunology, Neurobiology, Stem Cell Research, Angiogenesis, Oncology
• Product Line: Gibco™
• Purity or Quality Grade: ≥98% by SDS-PAGE

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov