Invitrogen™ Human PDGF-C ELISA Kit

Catalog No.
EH365RBX10	10 x 96 Tests; HRP

Catalog No. EH365RBX10 Supplier Invitrogen™ Supplier No. EH365RBX10

Description

ELISA

Human PDGF-C quantitates human PDGF-C in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PDGF-C.

Sphingosine Kinase 2 (Sphk2) catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cellcell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).

Specifications

• Accession Number: Q9NRA1
• Assay Range: 0.81-200 ng/mL
• Assay Sensitivity: 0.81 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110064L01Rik; AI647969; C76851; fallotein; MGC102297; Pdgfc; PDGF-C; PDGFC latent form; PDGFC receptor-binding form; platelet derived growth factor C; platelet-derived growth factor C; Platelet-derived growth factor C, latent form; Platelet-derived growth factor C, receptor-binding form; platelet-derived growth factor, C polypeptide; rScdfg; Scdgf; secretory growth factor-like protein; spinal cord-derived growth factor; UNQ174/PRO200; VEGF-E1110064L01Rik; AI647969; C76851; fallotein; MGC102297; Pdgfc; PDGF-C; PDGFC latent form; PDGFC receptor-binding form; platelet derived growth factor C; platelet-derived growth factor C; Platelet-derived growth factor C, latent form; Platelet-derived growth factor C, receptor-binding form; platelet-derived growth factor, C polypeptide; rScdfg; Scdgf; secretory growth factor-like protein; spinal cord-derived growth factor; UNQ174/PRO200; VEGF-E;
• Gene ID (Entrez): 56034
• Gene Symbol: PDGFC
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: PDGF-C
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min