Gibco™ Human PECAM-1 Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human PECAM-1 is a 572 amino acid glycoprotein comprising the extracellular domain of PECAM-1. Monomeric glycosylated PECAM-1 migrates at an apparent molecular weight of approximately 80.0-95.0 kDa by SDS-PAGE analysis under reducing conditions. The calculated molecular weight of Recombinan Human PECAM-1 is 64.3 kDa. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Specifications

• Accession Number: P16284
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 5175
• Molecular Weight (g/mol): 64.3 kDa
• Name: Human PECAM-1
• Quantity: 100 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule
• Common Name: CD31 (PECAM-1)
• Gene Symbol: Pecam1
• Biological Activity: Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: ENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQLLV EGVPSPRVTL DKKEAIQGGI VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQVTHLAQEF PEIIIQKDKA IVAHNRHGNK AVYSVMAMVE HSGNYTCKVE SSRISKVSSI VVNITELFSK PELESSFTHL DQGERLNLSC SIPGAPPANF TIQKEDTIVS QTQDFTKIAS KSDSGTYICT AGIDKVVKKS NTVQIVVCEM LSQPRISYDA QFEVIKGQTI EVRCESISGT LPISYQLLKT SKVLENSTKN SNDPAVFKDN PTEDVEYQCV ADNCHSHAKM LSEVLRVKVI APVDEVQISI LSSKVVESGE DIVLQCAVNE GSGPITYKFY REKEGKPFYQ MTSNATQAFW TKQKASKEQE GEYYCTAFNR ANHASSVPRS KILTVRVILA PWK
• Content And Storage: -20°C
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 97% by SDS-PAGE gel and HPLC analyses.