Invitrogen™ Human PIVKA-II ELISA Kit

Catalog No.	Quantity
EEL206	96 Tests

Catalog No. EEL206 Supplier Invitrogen™ Supplier No. EEL206

Description

ELISA

Human PIVKA-II ELISA Kit quantifies DCP in serum and plasma. The Human PIVKA-II solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II), also known as des-gamma-carboxy prothrombin (DCP), is an abnormal form of prothrombin produced primarily by liver tissues under conditions of vitamin K deficiency or antagonism. This immature prothrombin variant lacks sufficient coagulation activity and is prominently overexpressed in hepatocellular carcinoma (HCC) cells, serving as a crucial biomarker for screening and diagnosing HCC. PIVKA-II also appears in some cases of gastric cancer, albeit less frequently. Elevated serum levels of PIVKA-II are not exclusive to malignancies; they can also be seen in non-HCC conditions, which necessitates differential diagnostic strategies. The utility of PIVKA-II is further underscored by its ability to predict extrahepatic metastases in early HCC, supporting its role in comprehensive patient evaluation and monitoring.

Specifications

• Accession Number: P00734
• Assay Range: 0.16-10 ng/mL
• Assay Sensitivity: 0.09 ng/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Activation peptide fragment 1; Activation peptide fragment 2; Cf2; Cf-2; coagulation factor 2; coagulation factor II; coagulation factor II (thrombin); coagulation factor II, thrombin; F2; FII; PIVKA-II; prepro-coagulation factor II; Protein Induced by Vitamin K Absence or Antagonist-II; prothrombin; prothrombin B-chain; PT; RPRGL2; serine protease; THPH1; thrombin; Thrombin heavy chain; Thrombin light chainActivation peptide fragment 1; Activation peptide fragment 2; Cf2; Cf-2; coagulation factor 2; coagulation factor II; coagulation factor II (thrombin); coagulation factor II, thrombin; F2; FII; PIVKA-II; prepro-coagulation factor II; Protein Induced by Vitamin K Absence or Antagonist-II; prothrombin; prothrombin B-chain; PT; RPRGL2; serine protease; THPH1; thrombin; Thrombin heavy chain; Thrombin light chain;
• Gene ID (Entrez): 2147
• Gene Symbol: F2
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis, Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Shipping Condition: Wet Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: DCP
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min