Invitrogen™ Human PLA2G2A ELISA Kit

Catalog No.	Quantity
EH369RBX10	10 x 96 Tests

Catalog No. EH369RBX10 Supplier Invitrogen™ Supplier No. EH369RBX10

Description

ELISA

Human PLA2G2A quantitates human PLA2G2A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PLA2G2A.

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.

Specifications

• Accession Number: P14555
• Assay Range: 32-8000 pg/mL
• Assay Sensitivity: 32 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: EC 3.1.1.4; EF; Enhancing factor; eted enzyme type IIA phospholipase A2; GIIC sPLA2; Group IIA phospholipase A2; modifier of Min1; Mom1; non-pancreatic secreted type II phospholipase A2; non-pancreatic secretory phospholipase A2; NPS-PLA2; phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2 group IIA; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; Pla2; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLA2S; PLAS1; platelet phospholipase A2; RASF-A; secretory group II phospholipase A2; sPLA2; sPla2-IIAEC 3.1.1.4; EF; Enhancing factor; eted enzyme type IIA phospholipase A2; GIIC sPLA2; Group IIA phospholipase A2; modifier of Min1; Mom1; non-pancreatic secreted type II phospholipase A2; non-pancreatic secretory phospholipase A2; NPS-PLA2; phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2 group IIA; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; Pla2; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLA2S; PLAS1; platelet phospholipase A2; RASF-A; secretory group II phospholipase A2; sPLA2; sPla2-IIA;
• Gene ID (Entrez): 5320
• Gene Symbol: PLA2G2A
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: PLA2G2A
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min