Gibco™ Human Plateable Hepatocytes, Induction Qualified

Catalog No.	Quantity
HMCPIS	1.5 mL vial

Catalog No. HMCPIS Supplier Gibco™ Supplier No. HMCPIS

Description

Human Plateable Hepatocytes, Induction Qualified

These plateable cryopreserved human hepatocytes are inducible and suitable for use in experiments monitoring in vitro enzyme induction.

Cells per vial: 4-8 million

The potentials of known clinically relevant enzyme inducers represented by 3-methylcholanthrene (3-MC, a prototypical CYP1A enzyme inducer, 3μM), phenobarbital (PB, a prototypical CYP2B inducer, 1000μM) and rifampicin (RIF, a prototypical CYP3A enzyme inducer, 10μM) have been assessed to verify that each lot is inducible.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

Phase I and II enzyme activities

Proper morphology

Viabilities generally >75%

Attachment efficiency

Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

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Specifications

• Content And Storage: Store in a cryostorage dewar or -135°C freezer.
• Cell Type: Hepatocytes
• Culture Type: Mammalian Cell Culture
• Form: Cryopreserved
• Species: Human
• Donor Source: Single Donor
• No. of Cells: 4-8 x 10^6
• Product Line: Gibco
• Product Type: Hepatocytes
• Quantity: 1.5 mL vial

Frequently Asked Questions (FAQs)

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.

Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984

Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.

What should I plate hepatocyte cells on?

Hepatocytes need to be plated on collagen I-coated culture vessels.

I am looking for hepatocytes with particular characteristics. Who should I contact?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require cells with particular P450 values or donor specifications, please contact our Hepatic Biology Tech Support Team at hepaticproducts@invitrogen.com to help with specific lot recommendations.

What is a service level agreement (SLA) and do I need one when working with human tissue?

A service level agreement (SLA) is required ONLY if you are working in a laboratory in the European Union. An SLA is designed to comply with the UK's Human Tissue Authority (HTA) regulations and outlines the scope of use for human biologicals.

Are there guidelines for the procurement of human liver tissue for cryopreserved human hepatocytes?

Yes, we comply with country-specific legal and ethical standards for procurement of human liver tissue, including the global ICH Guidelines, and the US's HIPAA, Uniform Anatomical Gift Act, National Organ Transplant Act, and Hospital Internal Review Board (IRB) approval processes.

Where do you get liver tissue for cryopreserved human hepatocytes?

We source liver tissue from a network of hospitals in North America that perform surgical resections on living donors, as well as from deceased donors whose organs were rejected for transplant.

Will I be able to transfect my primary hepatocyte cells if I use an overlay?

No. The overlay will interfere with transfection. For transfection assays, we recommend that you perform the transfection first, and then proceed to overlay the cells.

For Research Use Only. Not for use in diagnostic procedures.