Invitrogen™ Human Podocalyxin ELISA Kit

Description

ELISA

Human Podocalyxin quantitates human Podocalyxin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Podocalyxin.

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

Specifications

• Accession Number: AW121214; GCTM-2 antigen; Gp200; Ly102; MGC138240; PC; PCLP; PCLP antibody; Pclp1; PCLP-1; PCLP1 antibody; PODL x 2; podocalyxin; podocalyxin like; podocalyxin-like; podocalyxin-like protein 1; podolyxin; Podxl
• Assay Range: 22 to 5,000 pg/mL
• Assay Sensitivity: 22 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5420
• Gene Symbol: PODXL
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Podocalyxin (PODXL)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins