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  5. Invitrogen™ Human PON1 ELISA Kit 

Invitrogen™ Human PON1 ELISA Kit

Catalog No. EH376RBX5
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Description

ELISA

Human PON1 quantitates human PON1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PON1.

The paraoxonase (PON) gene-family in humans has three members, PON1, PON2, and PON3, aligned next to each other on the long arm of chromosome 7q21.3-22.1. PON1 of these is synthesized primarily in the liver and a portion is secreted into the plasma, where it is associated with high density lipoproteins (HDL). And It hydrolyzes the active metabolites of several other organophosphorus insecticides (e.g., chlorpyrifos oxon, diazoxon), as well as nerve agents such as sarin, soman and VX (lethal nerve agent). The PON1 polymorphism may be responsible for neurodegeneration and considered to be an independent risk factor for Parkinson's disease.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number A esterase 1; A-esterase 1; aromatic esterase 1; arylesterase 1; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; esterase A; K-45; MVCD5; paraoxonase 1; paraoxonase B-type; Pon; PON 1; PON1; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; Serum paraoxonase/arylesterase 1
Assay Range 0.2 to 50 ng/mL
Assay Sensitivity 0.2 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5444
Gene Symbol PON1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target PON1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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