Invitrogen™ Human PON1 ELISA Kit

Description

ELISA

Human PON1 quantitates human PON1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PON1.

The paraoxonase (PON) gene-family in humans has three members, PON1, PON2, and PON3, aligned next to each other on the long arm of chromosome 7q21.3-22.1. PON1 of these is synthesized primarily in the liver and a portion is secreted into the plasma, where it is associated with high density lipoproteins (HDL). And It hydrolyzes the active metabolites of several other organophosphorus insecticides (e.g., chlorpyrifos oxon, diazoxon), as well as nerve agents such as sarin, soman and VX (lethal nerve agent). The PON1 polymorphism may be responsible for neurodegeneration and considered to be an independent risk factor for Parkinson's disease.

Specifications

• Accession Number: A esterase 1; A-esterase 1; aromatic esterase 1; arylesterase 1; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; esterase A; K-45; MVCD5; paraoxonase 1; paraoxonase B-type; Pon; PON 1; PON1; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; Serum paraoxonase/arylesterase 1
• Assay Range: 0.2 to 50 ng/mL
• Assay Sensitivity: 0.2 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5444
• Gene Symbol: PON1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: PON1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins