Invitrogen™ Human PPAR gamma ELISA Kit

Catalog No.	Quantity
EEL176	96 Tests

Catalog No. EEL176 Supplier Invitrogen™ Supplier No. EEL176

Description

ELISA

The Human Peroxisome Proliferator Activated Receptor Gamma (PPARgamma) ELISA quantitates PPARgamma in serum, plasma and other biological fluids. Principle of the method The Human Peroxisome Proliferator Activated Receptor Gamma (PPAR gamma) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Specifications

• Accession Number: P37231
• Assay Range: 0.16-10 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CIMT1; GLM1; HUMPPARG; NR1C3; nuclear hormone receptor; nuclear hormone receptor; PPARG; Nuclear receptor subfamily 1 group C member 3; nuclear receptor transcription factor; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferative activated receptor, gamma; peroxisome proliferative activated receptor, gamma-2; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma; peroxisome proliferator activated receptor gamma 1; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activated receptor gamma-1; peroxisome proliferator activated receptor gamma-2; peroxisome proliferator activatived receptor gamma; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor; peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma 1a; peroxisome proliferator-activated receptor gamma 1-a; peroxisome proliferator-activated receptor gamma 1b; peroxisome proliferator-activated receptor gamma 1-b; peroxisome proliferator-activated receptor gamma 1c; peroxisome proliferator-activated receptor gamma 1d; peroxisome proliferator-activated receptor gamma 2; peroxisome proliferator-activated receptor-gamma; peroxisome proliferator-activated receptor-gamma 2; peroxisome proliferator-cctivated receptor gamma 1; PPAR gamma; PPAR gamma 1; PPAR gamma 3; Pparg; PPARG1; PPARG2; PPARgamma; PPAR-gamma; PPARgamma2; PPAR-gamma2; PPARy; proliferator activated gamma; transcription factorCIMT1; GLM1; HUMPPARG; NR1C3; nuclear hormone receptor; nuclear hormone receptor; PPARG; Nuclear receptor subfamily 1 group C member 3; nuclear receptor transcription factor; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferative activated receptor, gamma; peroxisome proliferative activated receptor, gamma-2; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma; peroxisome proliferator activated receptor gamma 1; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activated receptor gamma-1; peroxisome proliferator activated receptor gamma-2; peroxisome proliferator activatived receptor gamma; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor; peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma 1a; peroxisome proliferator-activated receptor gamma 1-a; peroxisome proliferator-activated receptor gamma 1b; peroxisome proliferator-activated receptor gamma 1-b; peroxisome proliferator-activated receptor gamma 1c; peroxisome proliferator-activated receptor gamma 1d; peroxisome proliferator-activated receptor gamma 2; peroxisome proliferator-activated receptor-gamma; peroxisome proliferator-activated receptor-gamma 2; peroxisome proliferator-cctivated receptor gamma 1; PPAR gamma; PPAR gamma 1; PPAR gamma 3; Pparg; PPARG1; PPARG2; PPARgamma; PPAR-gamma; PPARgamma2; PPAR-gamma2; PPARy; proliferator activated gamma; transcription factor;
• Gene ID (Entrez): 5468
• Gene Symbol: PPARG
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: PPAR gamma
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min