Invitrogen™ Human Presenilin 1 ELISA Kit

Catalog No.	Quantity
EH380RBX10	10 x 96 Tests

Catalog No. EH380RBX10 Supplier Invitrogen™ Supplier No. EH380RBX10

Description

ELISA

Human Presenilin 1 quantitates human Presenilin 1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Presenilin 1.

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.

Specifications

• Accession Number: P49768
• Assay Range: 10.5-2500 pg/mL
• Assay Sensitivity: 10.5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AD3; Ad3h; Alzheimer Disease 3; FAD; Minilin; presenilin 1; presenilin 1 (Alzheimer disease 3); presenilin1; presenilin-1; Presenilin-1 CTF subunit; Presenilin-1 CTF12; Presenilin-1 NTF subunit; Protein S182; PS1; PS-1; PS1-CTF12; PSEN1; Psnl1; S182; S182 protein; Senilin 1AD3; Ad3h; Alzheimer Disease 3; FAD; Minilin; presenilin 1; presenilin 1 (Alzheimer disease 3); presenilin1; presenilin-1; Presenilin-1 CTF subunit; Presenilin-1 CTF12; Presenilin-1 NTF subunit; Protein S182; PS1; PS-1; PS1-CTF12; PSEN1; Psnl1; S182; S182 protein; Senilin 1;
• Gene ID (Entrez): 5663
• Gene Symbol: PSEN1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Presenilin 1
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min