Invitrogen™ Human Presenilin 2 ELISA Kit

Description

ELISA

Human Presenilin 2 quantitates human Presenilin 2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Presenilin 2.

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified.

Specifications

• Accession Number: AD3L; AD3LP; AD4; Ad4h; AD5; Alg3; ALG-3; Alzheimer disease 4; CMD1V; E5-1; presenilin 2; presenilin-2; Presenilin-2 CTF subunit; Presenilin-2 NTF subunit; PS2; Ps-2; PSEN 2; Psen2; Psnl2; STM2; STM-2
• Assay Range: 0.2 to 50 ng/mL
• Assay Sensitivity: 0.2 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5664
• Gene Symbol: PSEN2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: PSEN2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins