Invitrogen™ Human proGRP ELISA Kit

Catalog No.	Quantity
EEL185	96 Tests

Catalog No. EEL185 Supplier Invitrogen™ Supplier No. EEL185

Description

ELISA

The Human Pro Gastrin Releasing Peptide (ProGRP) ELISA quantitates ProGRP in serum, plasma and other biological fluids. Principle of the method The Human ProGRP solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

ProGRP, short for Pro-gastrin-releasing peptide, is a precursor protein that gives rise to the gastrin-releasing peptide (GRP) hormone. It plays a role in various physiological processes, particularly in the regulation of neuroendocrine functions. Functionally, proGRP is involved in the control of cell growth, neurotransmission, and the regulation of the release of gastrointestinal hormones. It acts as a neuropeptide and can influence the secretion of gastrin and other gastrointestinal peptides. Additionally, proGRP has been implicated in the development and progression of certain cancers, particularly small cell lung cancer. Structurally, proGRP is a protein that consists of a chain of amino acids. It undergoes post-translational processing to generate the active form of GRP, which is a shorter peptide consisting of 27 amino acids. Localization-wise, proGRP is primarily produced in the nervous system, particularly in neurons of the central and peripheral nervous systems. It is also found in certain endocrine cells of the gastrointestinal tract and in some neuroendocrine tumors.

Specifications

• Accession Number: P07492
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: pro-grppro-grp;
• Gene ID (Entrez): 2922
• Gene Symbol: grp
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: proGRP
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min