Invitrogen™ eBioscience™ Human Resistin Platinum ELISA Kit 

For the quantitative detection of human Resistin. Resistin is a hormone secreted by adipose tissue.

Manufacturer:  Invitrogen™ BMS2040

Catalog No. 50-179-46

Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Resistin
Biotin-Conjugate anti-human Resistin polyclonal antibody
Streptavidin-HRP
Human Resistin Standard lyophilized, 4000pg/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.

Specifications

• Accession Number: Q9HD89
• Conjugate: Biotin
• Gene ID (Entrez): 56729
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 tests
• Sensitivity: 3.1 pg/mL
• Sample Size: 50μL (1:10 prediluted)
• Species: Human
• Test Time: 1 hr 20 min
• Target: Human
• Synonym: Cysteine-rich secreted protein FIZZ3, ADSF, RETN1
• Intraassay CV: 5.1%

• Assay Range: 31-2,000 pg/mL
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA
• Regulatory Status: RUO
• Sample Type: Plasma, Serum, Supernatants
• Sample Volume: Plasma (15μL), Serum (15μL), Supernatants (50μL)
• Storage Requirements: 2-8°C
• Total Assay Time: 3 hr 10 min
• Gene Symbols: RETN
• Interassay CV: 8.1%
• Label or Dye: HRP

For Research Use Only