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Invitrogen™ Human RNF12 (aa 180-323) Control Fragment Recombinant Protein

Catalog No. RP92141
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RP92141 100 μL
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Catalog No. RP92141 Supplier Invitrogen™ Supplier No. RP92141
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Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (76%), Rat (76%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-82644. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

RNF12 is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9NVW2
Concentration ≥5.0 mg/mL
For Use With (Application) Neutralization, Control
Formulation PBS, 1M urea with no preservative; pH 7.4
Gene ID (Entrez) 51132
Name Human RNF12 (aa 180-323) Control Fragment
pH Range 7.4
Purification Method Purified
Quantity 100 μL
Storage Requirements -20°C, Avoid Freeze/Thaw Cycles
Regulatory Status RUO
Gene Alias AL022832; AW743871; CTD-2530H13.3; DKFZp686N06224; E3 ubiquitin-protein ligase RLIM; E3 ubiquitin-protein ligase RNF12; FLJ25923; FLJ41093; FLJ42887; FLJ45986; Ha1r; Hoxa1 regulated; LIM domain-interacting RING finger protein; MGC15161; NY-REN-43; renal carcinoma antigen NY-REN-43; RING finger LIM domain-binding protein; ring finger protein 12; ring finger protein, LIM domain interacting; ring zinc finger LIM domain binding protein; ring zinc finger protein NY-REN-43antigen; RING-type E3 ubiquitin transferase RLIM; Rlim; R-LIM; Rlim1; RNF12
Common Name RNF12
Gene Symbol RLIM
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence RSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDL
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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