Invitrogen™ Human ROBO3 ELISA Kit

Catalog No.	Quantity
EH393RBX10	10 x 96 Tests

Catalog No. EH393RBX10 Supplier Invitrogen™ Supplier No. EH393RBX10

Description

ELISA

Human ROBO3 quantitates human ROBO3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ROBO3.

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla.

Specifications

• Accession Number: Q96MS0
• Assay Range: 15-0.06 ng/mL
• Assay Sensitivity: 0.06 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: FLJ21044; HGPPS; HGPS; RBIG1; retinoblastoma inhibiting gene 1; retinoblastoma-inhibiting gene 1 protein; Rig1; Rig-1; ROBO3; Robo3a; Robo3b; roundabout guidance receptor 3; roundabout homolog 3; roundabout homolog 3 (Drosophila); roundabout, axon guidance receptor, homolog 3; roundabout, axon guidance receptor, homolog 3 (Drosophila); roundabout-like protein 3FLJ21044; HGPPS; HGPS; RBIG1; retinoblastoma inhibiting gene 1; retinoblastoma-inhibiting gene 1 protein; Rig1; Rig-1; ROBO3; Robo3a; Robo3b; roundabout guidance receptor 3; roundabout homolog 3; roundabout homolog 3 (Drosophila); roundabout, axon guidance receptor, homolog 3; roundabout, axon guidance receptor, homolog 3 (Drosophila); roundabout-like protein 3;
• Gene ID (Entrez): 64221
• Gene Symbol: Robo3
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: ROBO3
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min