Invitrogen™ Human RPS10 (aa 122-143) Control Fragment Recombinant Protein

Catalog No.	Quantity
RP99597	100 μL

Catalog No. RP99597 Supplier Invitrogen™ Supplier No. RP99597

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61645. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S10 (RPS10) is a 165 amino acid component of the small ribosomal 40S subunit. While generally found in the cytoplasm, RPS10 can localize to the granular component (GC) region of the nucleolus. For this to occur, methylation by PRMT5 is required as well as an interaction with NPS1. Defects in RPS10 are the cause of Diamond-Blackfan anemia type 9 (DBA9). It is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy.

Specifications

• Accession Number: P46783
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Neutralization, Control
• Formulation: PBS, 1M urea with no preservative; pH 7.4
• Gene ID (Entrez): 6204
• Name: Human RPS10 (aa 122-143) Control Fragment
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μL
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 2210402A09Rik; 40S ribosomal protein S10; DBA9; ribosomal protein S10; RP11-375E1__A.4; Rps10; S10; Small ribosomal subunit protein eS10
• Common Name: RPS10
• Gene Symbol: Rps10
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: ADRDTYRRSAVPPGADKKAEAG
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining