Human RTN4R Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of RTN4R in Tissue homogenates cell lysates and other biological fluids It provides a detection range of 0 32-20 ng/mL with a sensitivity of 0 122 ng/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RTN4R Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RTN4R Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Human RTN4R biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color