Human S100A6 Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of S100A6 in serum plasma tissue homogenates cell lysates cell culture supernates and other biological fluids It provides a detection range of 78 13-5000 pg/mL with a sensitivity of 30 pg/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A6 Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A6 Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Human S100A6 biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color