Invitrogen™ Human SARS-CoV-2 N ELISA Kit

Catalog No.	Quantity
EH490RB	96 Tests

Catalog No. EH490RB Supplier Invitrogen™ Supplier No. EH490RB

Description

ELISA

The Human SARS-CoV-2 Nucleocapsid ELISA quantitates Nucleocapsid in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Human Nucleocapsid. Principle of the method The Human SARS-CoV-2 Nucleocapsid solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). The nucleocapsid protein (N) was one of the structural proteins of SARS-CoV (N, S, sE, M). The severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein is the most abundant protein in the virus-infected cells. The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene, and nonsynonymous mutations developed in the S protein as the SARS-CoV epidemic developed, reports have shown that the crystal structure of the SARS-CoV-2 nucleocapsid protein is similar to those of previously described coronavirus N proteins, but their surface electrostatic potential characteristics are distinct.

Specifications

• Assay Range: 0.069-50 ng/mL
• Assay Sensitivity: 0.069 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Nasopharyngeal Swab, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Coronavirus; COVID; COVID 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Nucleocapsid; SARS-CoV2; SARS-CoV-2Coronavirus; COVID; COVID 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Nucleocapsid; SARS-CoV2; SARS-CoV-2;
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 25 μL, Serum 25 μL, Supernatant 25 μL
• Shipping Condition: Wet Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: SARS Coronavirus Nucleocapsid
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min