Invitrogen™ Human Semaphorin 4D ELISA Kit

Catalog No.	Quantity
EH405RBX5	5 x 96 Tests
EH405RBX10	10 x 96 Tests
EH405RB	96 Tests

Catalog No. EH405RBX5 Supplier Invitrogen™ Supplier No. EH405RBX5

Description

ELISA

Human Semaphorin 4D quantitates human Semaphorin 4D in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Semaphorin 4D.

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Specifications

• Accession Number: A8; BB18; C9orf164; CD100; coll-4; GR3; M-Sema G; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4 D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4 D; sema J; Sema4d; Semacl2; SEMAJ; semaphorin 4 D; semaphorin H; semaphorin-4 D; Semaphorin-C-like 2; semaphorin-J; Semcl2
• Assay Range: 0.62 to 150 ng/mL
• Assay Sensitivity: 0.62 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 10507
• Gene Symbol: Sema4d
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD100 (SEMA4D)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins