Invitrogen™ Human Semaphorin 6D ELISA Kit

Catalog No.	Quantity
EH409RBX10	10 x 96 Tests

Catalog No. EH409RBX10 Supplier Invitrogen™ Supplier No. EH409RBX10

Description

ELISA

Human Semaphorin 6D quantitates human Semaphorin 6D in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Semaphorin 6D.

Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphoring domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. SEMA6D is a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Six transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner.

Specifications

• Accession Number: Q8NFY4
• Assay Range: 1.64-400 ng/mL
• Assay Sensitivity: 1.64 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110067B02Rik; AA409156; D330011G23; FLJ11598; hypothetical protein LOC406272; KIAA1479; KIAA1479FLJ11598; mKIAA1479; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D; Sema6d; sema6d protein; semaphorin 6D; semaphorin-6D; wu:fi06a12; wu:fi76a10; zgc:561701110067B02Rik; AA409156; D330011G23; FLJ11598; hypothetical protein LOC406272; KIAA1479; KIAA1479FLJ11598; mKIAA1479; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D; Sema6d; sema6d protein; semaphorin 6D; semaphorin-6D; wu:fi06a12; wu:fi76a10; zgc:56170;
• Gene ID (Entrez): 80031
• Gene Symbol: SEMA6D
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: SEMA6D
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min