Invitrogen™ Human Serpin A3/Alpha-1-Antichymotrypsin ELISA Kit

Catalog No.	Quantity
EH411RBX5	5 x 96 Tests
EH411RBX10	10 x 96 Tests
EH411RB	96 Tests

Catalog No. EH411RBX5 Supplier Invitrogen™ Supplier No. EH411RBX5

Description

ELISA

Human Serpin A3/Alpha-1-Antichymotrypsin quantitates human Serpin A3/Alpha-1-Antichymotrypsin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Serpin A3/Alpha-1-Antichymotrypsin.

SERPINA encodes Alpha 1-ACT. It is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain. Regulation of the serine proteases and serine protease inhibitors plays an important role in neuromuscular differentiation. Prostate specific antigen (PSA), a chymotrypsin-like serine protease, is predominantly complexed to Alpha-1-ACT.

Specifications

• Accession Number: P01011
• Assay Range: 0.137-100 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AACT; ACT; alpha-1-antichymotrypsin; Alpha-1-antichymotrypsin His-Pro-less; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; Growth inhibiting protein 24; Growth inhibiting protein 25; growth-inhibiting protein 24; growth-inhibiting protein 25; MGC88254; Serine (or cysteine) proteinase inhibitor clade A member 3; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin A3; serpin family A member 3; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; SERPINA3AACT; ACT; alpha-1-antichymotrypsin; Alpha-1-antichymotrypsin His-Pro-less; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; Growth inhibiting protein 24; Growth inhibiting protein 25; growth-inhibiting protein 24; growth-inhibiting protein 25; MGC88254; Serine (or cysteine) proteinase inhibitor clade A member 3; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin A3; serpin family A member 3; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; SERPINA3;
• Gene ID (Entrez): 12
• Gene Symbol: SERPINA3
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: SERPINA3
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min