Invitrogen™ Human Serpin F1 ELISA Kit

Description

ELISA

Human Serpin F1 quantitates human Serpin F1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Serpin F1.

PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors, such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity (1) should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of vasculature-related neurodegenerative diseases, such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers.

Specifications

• Accession Number: AI195227; alpha-2 antiplasmin; caspin; Cell proliferation-inducing gene 35 protein; Dmrs91; EPC 1; EPC1; EPC-1; OI12; OI6; PEDF; Pedfl; PIG 35; PIG35; pigment epithelium derived factor; pigment epithelium-derived factor; Sdf3; SDF-3; serine (or cysteine) peptidase inhibitor, clade F, member 1; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serine (or cysteine) proteinase inhibitor, clade F), member 1; serine (or cysteine) proteinase inhibitor, clade F, member 1; serpin F1; serpin family F member 1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serpin peptidase inhibitor, clade F, member 1; SERPINF 1; SERPINF1; serpin-F1; sserpin family F member 1; stromal cell derived factor 3; stromal cell-derived factor 3; testis tissue sperm-binding protein Li 70 n
• Assay Range: 0.6 to 160 μg/mL
• Assay Sensitivity: 0.6 μg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5176
• Gene Symbol: SERPINF1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: PEDF
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins