Invitrogen™ Human sFRP-1 ELISA Kit

Catalog No.	Quantity
EH418RBX10	10 x 96 Tests

Catalog No. EH418RBX10 Supplier Invitrogen™ Supplier No. EH418RBX10

Description

ELISA

Human sFRP-1 quantitates human sFRP-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human sFRP-1.

Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Specifications

• Accession Number: Q8N474
• Assay Range: 0.78-50 ng/mL
• Assay Sensitivity: 0.78 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 2210415K03Rik; AW011917; AW107218; AW742929; frizzled-related protein; FRP; FRP1; FRP-1; FrzA; SARP2; SARP-2; Secreted apoptosis-related protein 2; secreted frizzled related protein 1; secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1; SFRP1; sFRP-12210415K03Rik; AW011917; AW107218; AW742929; frizzled-related protein; FRP; FRP1; FRP-1; FrzA; SARP2; SARP-2; Secreted apoptosis-related protein 2; secreted frizzled related protein 1; secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1; SFRP1; sFRP-1;
• Gene ID (Entrez): 6422
• Gene Symbol: SFRP1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: SFRP1
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min