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Invitrogen™ ICAM-1 (Soluble) Human ELISA Kit

For the quantitative detection of human sICAM-1.

Manufacturer:  Invitrogen™ BMS201TEN

Encompass

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Catalog No. 50-178-94


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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-1
HRP Conjugate anti-human sICAM-1 monoclonal antibody
Human sICAM-1 Standard, 100ng/mL
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

Description

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
Specifications

Specifications

HRP
ELISA Kit
P05362
ELISA
Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, Detection Antibody Conjugate: anti-rabbit-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
1 hr. 20 min.
Human
7.7%
10 x 96 Tests
Plasma, Serum, Supernatant
BB2,CD54,P3.58
Colorimetric Microplate Reader
HRP
6.25-100 ng/mL
3383
RUO
2°C to 8°C
1 hr. 10 min.
Intercellular adhesion molecule 1, CD54 antigen
4.1%
Human
2.2 ng/mL
SDS
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