Invitrogen™ Human SIRP-gamma ELISA Kit

Description

ELISA

SIRP gamma (CD172g) recognizes a 55 kDa signal regulatory protein which is a member of the immunoglobulin gene superfamily. SIRP gamma is expressed on most T lymphocytes and a subset of B-cells but is absent on myeloid cells. CD172g has a truncated cytoplasmic tail, which is similar to SIRP beta, but unlike SIRP beta CD172g does not require DAP12 for expression at the cell surface. CD172g, like SIRP alpha, binds to CD47 inducing apoptosis. SIRP gamma is also involved in the negative regulation of receptor tyrosine kinase-coupled signalling.

Specifications

• Accession Number: Q9P1W8
• Assay Range: 0.41 to 100 ng/mL
• Assay Sensitivity: 0.41 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 55423
• Gene Symbol: SIRPG
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: SIRP gamma
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins