Invitrogen™ Human SIRT1 ELISA Kit

Catalog No.	Quantity
EH427RBX10	10 x 96 Tests

Catalog No. EH427RBX10 Supplier Invitrogen™ Supplier No. EH427RBX10

Description

ELISA

Human SIRT1 quantitates human SIRT1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human SIRT1.

NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

Specifications

• Accession Number: Q96EB6
• Assay Range: 1.23-300 ng/mL
• Assay Sensitivity: 1.23 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 75SirT1; AA673258; hSIR2; hSIRT1; mSIR2a; NAD-dependent deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; OTTHUMP00000198111; OTTHUMP00000198112; Regulatory protein SIR2 homolog 1; RP11-57G10.3; SIR2; Sir2a; SIR2alpha; SIR2L1; sir2-like 1; SIR2-like protein 1; SIRT 1; SIRT1; SIRT-1; SirtT1 75 kDa fragment; sirtuin 1; sirtuin 1 ((silent mating type information regulation 2, homolog) 1; sirtuin type 175SirT1; AA673258; hSIR2; hSIRT1; mSIR2a; NAD-dependent deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; OTTHUMP00000198111; OTTHUMP00000198112; Regulatory protein SIR2 homolog 1; RP11-57G10.3; SIR2; Sir2a; SIR2alpha; SIR2L1; sir2-like 1; SIR2-like protein 1; SIRT 1; SIRT1; SIRT-1; SirtT1 75 kDa fragment; sirtuin 1; sirtuin 1 ((silent mating type information regulation 2, homolog) 1; sirtuin type 1;
• Gene ID (Entrez): 23411
• Gene Symbol: SIRT1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: SIRT1
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min