Invitrogen™ Human SOX9 ELISA Kit

Description

ELISA

Human SOX9 quantitates human SOX9 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human SOX9.

SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

Specifications

• Accession Number: 2010306G03Rik; AV220920; CMD1; CMPD1; mKIAA4243; SOX 9; So x 9; SOX-9; SRA1; SRX x 2; SRXY10; SRY (sex determining region Y)-box 9; SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal); SRY (sex determining region Y)-bo x 9; SRY (sex-determining region Y)-box 9 protein; SRY box 9; SRY-box 9; SRY-Box 9 ; SRY-box containing 9; SRY-box containing gene 9; SRY-box containing protein 9; SRY-related HMG-box, gene 9; Transcription factor SOX-9
• Assay Range: 0.41 to 100 ng/mL
• Assay Sensitivity: 0.41 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6662
• Gene Symbol: SOX9
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: SOX9
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov