Invitrogen™ Human SPAG5 (aa 155-298) Control Fragment Recombinant Protein

Catalog No.	Quantity
RP108753	100 μL

Catalog No. RP108753 Supplier Invitrogen™ Supplier No. RP108753

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (41%), Rat (41%). This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

Specifications

• Accession Number: Q96R06
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Neutralization, Control
• Formulation: PBS, 1M urea with no preservative; pH 7.4
• Gene ID (Entrez): 10615
• Name: Human SPAG5 (aa 155-298) Control Fragment
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μL
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: AI874642; Astrin; D11Bhm180e; Deepest; hMAP126; MAP126; Mastrin; microtubule-associated protein; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; mitotic spindle-associated protein p126; S17; Spag5; sperm associated antigen 5; sperm-associated antigen 5
• Common Name: SPAG5
• Gene Symbol: SPAG5
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: TNSISLNGPLRTDDLVREEVAPCMGDRFSEVAAVSEKPIFQESPSHLLEESPPNPCSEQLHCSKESLSSRTEAVREDLVPSESNAFLPSSVLWLSPSTALAADFRVNHVDPEEEIVEHGAMEEREMRFPTHPKESETEDQALVS
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining