Invitrogen™ Human VAP-1 ELISA Kit

Catalog No.	Quantity
50-181-83	96 Tests
50-181-84	10 x 96 Tests

Catalog No. 50-181-83 Supplier Invitrogen™ Supplier No. BMS259

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sVAP-1
Biotin-Conjugate anti-human sVAP-1 monoclonal antibody
Streptavidin-HRP
Human sVAP-1 Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Human Vascular Adhesion Protein (Hu VAP-1) ELISA quantitates Hu VAP-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VAP-1. Principle of the method The Human VAP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been discribed and is located approximately 9-kb downstream.

Specifications

• Accession Number: Q16853
• Assay Range: 31.3 to 2,000 pg/mL
• Assay Sensitivity: 19 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 8639
• Gene Symbol: HPAO,SSAO,VAP-1,VAP1
• Interassay CV: 4.5%
• Intraassay CV: 3
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Vascular adhesion protein 1, copper-containing 3
• Target: AOC3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 10 mins