Invitrogen™ Human VEGF Receptor 1 (Soluble) ELISA Kit

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sVEGF-R1
Biotin-Conjugate anti-human sVEGF-R1 polyclonal antibody
Streptavidin-HRP
Human sVEGF-R1 Standard lyophilized, 20ng/mL upon reconstitution
Conjugate Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Human Vascular Endothelial Growth Factor Receptor 1 (Hu VEGFR1) ELISA quantitates Hu VEGFR1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGFR1. Principle of the method The Human VEGFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

FLT1 (VEGFR1) is a receptor tyrosine kinase which plays a critical role in angiogenesis. Active angiogenesis has been found to play important role in many pathological and physiological processes, such as tumor progression, diabetic retinopathy, rheumatoid arthritis and wound healing. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR.

Specifications

• Accession Number: P17948
• Assay Range: 0.16 to 10.0 ng/mL
• Assay Sensitivity: 0.03 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2321
• Gene Symbol: FLT,FLT-1,VEGFR-1,VEGFR1
• Interassay CV: 5.1%
• Intraassay CV: 5.5
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Vascular endothelial growth factor receptor1, FLT1
• Target: VEGF Receptor 1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins